ASTM D5712-2005e1 用改进半空细胞法分析天然橡胶及其制品中水可萃取蛋白质的标准试验方法
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【英文标准名称】:StandardTestMethodforAnalysisofAqueousExtractableProteininNaturalRubberandItsProductsUsingtheModifiedLowryMethod
【原文标准名称】:用改进半空细胞法分析天然橡胶及其制品中水可萃取蛋白质的标准试验方法
【标准号】:ASTMD5712-2005e1
【标准状态】:现行
【国别】:
【发布日期】:2005
【实施或试行日期】:
【发布单位】:美国材料与试验协会(US-ASTM)
【起草单位】:D11.40
【标准类型】:(TestMethod)
【标准水平】:()
【中文主题词】:蛋白质;天然橡胶;试验;分光光度法
【英文主题词】:aminoacidanalysis;Lowry;naturalrubberlatex;protein
【摘要】:Thistestmethod,forthedeterminationofproteinlevelsinNR,isprimarilyintendedtotestNRmaterialsforresidualproteincontent.Itisassumedthatallwhousethistestmethodwillbetrainedanalystscapableofperformingcommonlaboratoryproceduresskillfullyandsafely.Itisexpectedthatworkwillbeperformedinaproperlyequippedlaboratory.1.1Thistestmethodcoversananalyticaltestfordeterminingtheamountoftotalaqueousextractableproteinassociatedwithnaturalrubber(NR)anditsproducts.Watersolubleproteinsareextractedinabuffersolutionandthenprecipitatedtoconcentratethemandalsotoseparatethemfromwatersolublesubstancesthatmayinterferewiththedetermination.TheextractedproteinisredissolvedandquantifiedcolorimetricallybythemodifiedLowrymethodusingaproteinstandard.1.2Forthepurposeofthistestmethod,therangeofproteinmeasurementwillbebasedonthelimitofdetectionandquantitationandrecordedinmicrogramsperdm2NRspecimen.1.3Thetestmethodisdesignedtobeaccurateandcompatiblewiththeindustrialenvironment.1.4Stepsareincludedinthistestmethodtominimizetheeffectsofinterferingsubstances.1.5Itisrecognizedthatothermethodsfortheanalysisofleachableproteinsexistandthesemaybeusedforroutinequalitycontrolpurposesprovidedtheyhavebeenvalidatedandacorrelationestablishedagainstthereferencemethodspecifiedbythistestmethod.1.6ThevaluesstatedinSIunitsaretoberegardedasthestandard.Nootherunitsofmeasurementareincludedinthisstandard.1.7Thisstandarddoesnotpurporttoaddressallofthesafetyconcerns,ifany,associatedwithitsuse.Itistheresponsibilityoftheuserofthisstandardtoestablishappropriatesafetyandhealthpracticesanddeterminetheapplicabilityofregulatorylimitationspriortouse.
【中国标准分类号】:G40
【国际标准分类号】:83_040_10
【页数】:9P.;A4
【正文语种】:
【原文标准名称】:用改进半空细胞法分析天然橡胶及其制品中水可萃取蛋白质的标准试验方法
【标准号】:ASTMD5712-2005e1
【标准状态】:现行
【国别】:
【发布日期】:2005
【实施或试行日期】:
【发布单位】:美国材料与试验协会(US-ASTM)
【起草单位】:D11.40
【标准类型】:(TestMethod)
【标准水平】:()
【中文主题词】:蛋白质;天然橡胶;试验;分光光度法
【英文主题词】:aminoacidanalysis;Lowry;naturalrubberlatex;protein
【摘要】:Thistestmethod,forthedeterminationofproteinlevelsinNR,isprimarilyintendedtotestNRmaterialsforresidualproteincontent.Itisassumedthatallwhousethistestmethodwillbetrainedanalystscapableofperformingcommonlaboratoryproceduresskillfullyandsafely.Itisexpectedthatworkwillbeperformedinaproperlyequippedlaboratory.1.1Thistestmethodcoversananalyticaltestfordeterminingtheamountoftotalaqueousextractableproteinassociatedwithnaturalrubber(NR)anditsproducts.Watersolubleproteinsareextractedinabuffersolutionandthenprecipitatedtoconcentratethemandalsotoseparatethemfromwatersolublesubstancesthatmayinterferewiththedetermination.TheextractedproteinisredissolvedandquantifiedcolorimetricallybythemodifiedLowrymethodusingaproteinstandard.1.2Forthepurposeofthistestmethod,therangeofproteinmeasurementwillbebasedonthelimitofdetectionandquantitationandrecordedinmicrogramsperdm2NRspecimen.1.3Thetestmethodisdesignedtobeaccurateandcompatiblewiththeindustrialenvironment.1.4Stepsareincludedinthistestmethodtominimizetheeffectsofinterferingsubstances.1.5Itisrecognizedthatothermethodsfortheanalysisofleachableproteinsexistandthesemaybeusedforroutinequalitycontrolpurposesprovidedtheyhavebeenvalidatedandacorrelationestablishedagainstthereferencemethodspecifiedbythistestmethod.1.6ThevaluesstatedinSIunitsaretoberegardedasthestandard.Nootherunitsofmeasurementareincludedinthisstandard.1.7Thisstandarddoesnotpurporttoaddressallofthesafetyconcerns,ifany,associatedwithitsuse.Itistheresponsibilityoftheuserofthisstandardtoestablishappropriatesafetyandhealthpracticesanddeterminetheapplicabilityofregulatorylimitationspriortouse.
【中国标准分类号】:G40
【国际标准分类号】:83_040_10
【页数】:9P.;A4
【正文语种】:
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